LKK0120 Chemical Biology / Keemiline Bioloogia
In Y2020/2021, the course details are available on https://moodle.taltech.ee (to recieve a general password, please email Pavel; all slides, additional reading, assignments are in the Moodle; Tuesdays 12:00 till 13:30 SCI-120 and Wednesdays 13:45 till 15:15; experimental works are arranged separately).
In Y2019/2020, the course was not run because of COVID-19.
In Y2018/19, the course is will be jointly run with Dr Pirjo Spuul (Cell Biology) in Spring 2019 and will feature lab assignments (6 EAP).
In Y2017/18, the course was run in Fall 2017 as LKX0101 (3 EAP). For course materials, please visit http://old.taltech.ee/study-10 and use your username/password.
General, introduction, prebiotic chemistry (PNA etc.), DoS (cell painting, principal moment of inertia, Tanimoto similarity coefficient, Lipinski rules), BoS, Fluorine (incl. permeability, lipophilicity, P-gp, Caco-2, millicell), magic methyl effect.
The classics (BTS, blebbistatin, monostrol), cytoskeleton (intro), cell cycle (FACS).
Cytoskeleton (actin, myosin, cell polarisation and migration, chemotaxis, GTPases), chemical linkers (zero-length, non-zero-length, molecular rulers, spacers).
Cytoskeleton (MTs), PTMs (natural and unnatural), unnatural aminoacids, bioorthogonal chemistry (modifications of living cells, Staudinger reaction), biologicals (nanobodies, stability), hallmarks of cancer.
MT motors (kinesins, dyneins), cancer (types, molecular basis, stages), histological staining, fluorescent staining (organic fluorophores-chemistry).
Light microscopy (brightfield, darkfield, phase, DIC, sectioning), fluorescence microscopy (FISH, antibodies, DNA stains, organic fluorophores-classes, widefield, confocal, ExM).
Fluorescent proteins (chemistry, fusion proteins, FRET, FRAP, photoactivation, bioluminescence), superresolution techniques (3D SIM, STED, STORM, PALM), fluorescent proteins (photoconversion-chemistry), other techniques (TIRF, AFM, TEM, SEM, cryoEM).
The data on the rest of the meetings are in the password-protected study section (incl. mechanotransduction and microfluidics).